The Calprotectin Rapid Test Sheet (stool) is an immunochromatographic method for the qualitative detection of Calprotectin in human stool, which aids in the diagnosis of gastrointestinal inflammatory processes.
Calprotectin is a 24 kDa dimer of the calcium-binding proteins S100A8 and S100A9. Elevated levels of calprotectin indicate neutrophil infiltration into the intestinal mucosa, which occurs in inflammatory processes of the intestinal tract. The stool calprotectin content is a marker for the detection of intestinal inflammation. The advantage of calprotectin is that it is resistant to enzymatic degradation and can be easily detected in faeces.
The Calprotectin Rapid Test Card is a membrane-based immunochromatographic method for the qualitative detection of Calprotectin in human faeces. The membrane is coated with anti-Calprotectin antibody in the test (T) zone. During the test, the sample reacts with the particles coated with anti-Calprotectin antibody. The mixture, which is absorbed by capillary action, is chromatographed on the membrane and reacts with the anti-Calprotectin antibody to form a coloured strip. A coloured stripe appearing in the test (T) zone of the test sheet indicates a positive result and the absence of a coloured stripe indicates a negative result. To verify the procedure, a coloured stripe in the control (C) zone of the test sheet indicates correct operation of the test sheet, confirming that the sample volume and absorption were adequate.
