The FOB Rapid Test Sheet (faeces) is an immunochromatographic test for the qualitative detection of occult haemorrhage of human origin in faeces.
Summary:
Many diseases can cause invisible blood in the stool, also known as Fecal Occult Blood (FOB), Human Occult Blood, or Human Hemoglobin. In the early stages, gastrointestinal diseases such as colon cancer, polyps, colitis, diverticulitis, fissures do not cause visible symptoms, only occult bleeding. Traditional guaiacol-based detection is not sensitive and specific enough, and diet is mandatory before testing.1,2
The FOB Rapid Test Card (faeces) is a rapid qualitative test for the detection of small amounts of human blood in faeces. The test uses a dual antibody sandwich technique to selectively detect 40ng/ml of blood or 4.8µg/g faecal occult blood. Unlike the guaiacol procedure, diet is not required and diet does not affect the result.
Principle:
The FOB Rapid Test Plate (faecal) is a qualitative immunoassay, planar flow immunochromatographic method for the detection of blood from faeces. A membrane antihemoglobin antibody is recorded in the test zone. During the test, the sample reacts with particles containing anti-hemoglobin antibody. The mixture is chromatographed on the membrane by capillary uptake and forms a coloured strip in reaction with the fixed anti-hemoglobin antibodies. A coloured stripe in the test window indicates a positive result and a negative result in the absence of a coloured stripe. To verify the procedure, a coloured stripe appears in the control zone, confirming the correct operation of the test sheet, the correct sample volume and the absorption of the sample.
