Transferrin/FOB and Hb-Hp Combined Rapid Test Sheet is a (non-invasive) immunochromatographic test for the qualitative detection of human hemoglobin, transferrin and hemoglobin (Hb)/haptoglobin (Hp) complex from human stool, which aids in the diagnosis of gastrointestinal diseases associated with intestinal bleeding.
Colorectal cancer is a cancer of the colon or rectum that affects both men and women, most commonly in the over-50s. Occult blood in the stool is an important sign in the diagnostic evaluation of diseases where gastrointestinal bleeding of any aetiology is present, which is not only characteristic of colorectal cancer. The presence of human haemoglobin in faeces is not an appropriate screening test for gastric cancer, as human haemoglobin from the upper digestive tract is degraded in the intestinal tract (antigenicity is lost). The detection of transferrin in faeces, which is more stable in faeces than haemoglobin, offers an alternative method for the diagnosis of upper digestive tract disease.
The membrane test line region is coated with anti-hemoglobin antibody and anti-transferrin antibody. During the test, the sample reacts with particles coated with anti-hemoglobin antibody and/or anti-transferrin antibody. The mixture migrates up the membrane by capillary action and then reacts with the anti-hemoglobin and/or anti-transferrin antibody on the membrane, resulting in a coloured strip. The presence of a coloured stripe in the test line region indicates a positive result, whereas the absence of a coloured stripe indicates a negative result. To verify the procedure, a coloured stripe appears in the control zone, confirming the correct functioning of the test sheet, the correct sample volume and the absorption of the sample.
