H-FABP/Myog/CK-MB/Troponin I combined test sheet from blood/serum/plasma sample - 10pcs

Myoglobin (MYO), creatine kinase MB (CK-MB) and myocardial troponin I (cTnI) are proteins that are released and released into the bloodstream after damage to the heart. Myoglobin is a heme protein with a molecular weight of 17.8kDa, usually found in skeletal muscle and myocardium.1 Upon damage to muscle cells, the relatively small myoglobin is rapidly released and released into the bloodstream. Myoglobin levels increase measurably for 2-4 hours after infarction, reaching a peak within 9-12 hours after which they return to baseline within 24-36 hours.2,3 87.0 The enzyme CK-MB, with a molecular weight of kDa, is also present in myocardium. 4 Creatine kinase is a dimeric molecule whose two subunits, labelled 'M' and 'B' respectively, form three different isoenzymes: CK-MM, CK-BB and CK-MB. CK-MB is the isoenzyme of creatine kinase that plays the major role in the metabolism of myocardial tissue.5 CK-MB in the blood is detectable within 3-8 hours after the onset of myocardial infarction symptoms. It reaches peak blood levels within 9-30 hours and returns to baseline within 48-72 hours.6 Myocardial Troponin I, a protein of 22.5kDa molecular weight, is found in myocardium.7 Troponin I is part of a three-subunit complex with Troponin T and Troponin C. In addition to tropomyosin, this structural complex regulates the calcium-sensitive ATPase activity of actomyosin in striated and cardiac muscle.8 Troponin I is released into the blood within 4-6 hours after the onset of pain from cardiac injury. The release pattern of Troponin I is similar to CK-MB, but while CK-MB levels normalize within 72 hours, Troponin I levels remain elevated for 6-10 days and can be detected for a longer period after cardiac injury.

FABP is a newly introduced plasma marker for the detection of acute myocardial infarction (AMI). The plasma kinetics of FABP (15kD) are very similar to myoglobin, in that elevated plasma concentrations are detected within 2 hours post AMI and then usually normalise within 18-24 hours. The concentration of FABP in skeletal muscle is 20 times lower than in myocardial tissue, whereas myoglobin has the same content in cardiac and skeletal tissue, making FABP more cardiac specific than myoglobin. FABP-t is a useful biochemical marker for early assessment or exclusion of AMI. FABP also appears to be a useful plasma marker for estimating the extent of myocardial infarction. FABP is a suitable immunological assay for early detection of acute myocardial infarction, is immunogenic for antiserum production, and can be used as a bulk FABP standard for FABP biochemical and immunochemical assays.

The H-FABP and Myoglobin/CK-MB/Troponin I Combined Rapid Test Card (Whole Blood/Serum/Plasma) is a simple-to-perform test that uses a combination of antibody-coated particles and capture reagents for the qualitative detection of H-FABP, Myoglobin, CK-MB and myocardial Troponin I (cTnI) in whole blood, serum or plasma. The minimum detection levels are 8 ng/ml H-FABP, 50ng/ml myoglobin, 5ng/ml CK-MB and 0.5ng/ml Troponin I.