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Menopause is the permanent cessation of menstruation, which is not usually diagnosed until after the menstrual cycle has stopped. The 12 months leading up to menopause are called premenopause. Many women experience symptoms of this period, such as hot flushes, irregular menstrual cycles, sleep disturbances, vaginal dryness, baldness, anxiety, mood swings, short-term memory loss and fatigue. The symptoms of premenopause are caused by changes in the levels of hormones produced by the female body that regulate the menstrual cycle. As the body produces less and less of the hormone oestrogen, it increases the production of follicle stimulating hormone (FSH), which would normally regulate ovulation.
It follows that FSH testing can help determine the age of premenopause. Detecting that the age of premenopause has been reached allows appropriate steps to be taken to protect health and reduce the side effects of menopause, such as osteoporosis, high blood pressure, high cholesterol and increased risk of heart disease.
The FSH Rapid Menopause Test Sheet is a qualitative rapid test that detects FSH in urine with a sensitivity of 25 mIU/ml. The test uses a combination of monoclonal FSH antibody method to selectively detect elevated FSH levels. At the concentrations tested, the FSH One Step Menopause Test Card (Urine) does not cross-react with similarly structured glycoprotein hormones (hCG, LH, and TSH) even at high physiological concentrations.
The FSH Rapid Menopause Test Card is a qualitative, planar flow immunoassay for the qualitative detection of human follicle stimulating hormone (FSH) in urine to indicate the onset of female menopause. The test is based on a combination of antibodies and selectively detects elevated FSH levels using monoclonal anti-FSH antibodies. The test involves dropping a urine sample into the sample window of the test sheet and observing the formation of coloured bands.
The sample passes through the membrane and reacts with the coloured conjugate due to capillary action. Positive samples react with a specific antibody-FSH dye conjugate (antibody + colouring reagent) and form a coloured band across the membrane in the test zone and the rabbit antibody in the control zone of the membrane is released to the conjugate in the control zone. Two stripes will appear. If the stripe appearing in the test zone is the same or darker than the stripe appearing in the control zone, it indicates a positive result. If the stripe appearing in the test zone is paler than the stripe appearing in the control zone or does not appear at all, the result is considered negative.
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