The Clostridium difficile GDH +Toxin A +Toxin B Combined Rapid Test Kit (Faeces) is a rapid immunochromatographic immunoassay for the qualitative detection of Clostridium difficile GDH, Toxin A and Toxin B antigens in human faeces samples.
Conclusion:
Clostridium difficile is an anaerobic bacterium that exerts a pathogenic effect on the normal flora of the small intestine, especially following antibiotic treatment. Toxigenic strains of Clostridium difficile cause infection and diarrhoea and pseudomembranous colitis, which is a side-effect of antibiotics, and potentially fatal. Glutamate dehydrogenase enzyme (GDH) is a very effective antigenic marker of C. difficile proliferation, as all strains produce high levels of this enzyme.
The Clostridium difficile Toxin A +Toxin B Combined Rapid Test Kit (Faeces) allows the detection of GDH enzyme, in addition to C. difficile Toxin A and Toxin B, specifically in faecal samples.
Principle:
The Clostridium difficile Rapid Test Card isolates and detects three different antigens C. difficile GDH, Toxin A, Toxin B, on three different test strips in parallel on one test card in faeces.
C. difficile-specific GDH detection
In the test region, the membrane anti-C.diff. GDH antibody is coated. During testing, the sample reacts with the anti-C.diff.e-GDH antibody-coated particle. The mixture is chromatographed by capillary action of the membrane and reacts with the anti-C.diff. GDH antibody and forms a coloured band.
Specific detection of C. difficile Toxin A
In the test region, the membrane anti-C.diff. Toxin A antibody is coated on the test membrane. During testing, the sample reacts with the anti-C.diff. Toxin A antibody-coated particle. The mixture is chromatographed by capillary action of the membrane and reacts with the anti-C.diff. Toxin A antibody and forms a coloured band. The presence of a coloured line in the line of the test band indicates a positive result, the absence of a coloured line indicates a negative result.
Specific detection of C. difficile Toxin B
The test region in the membrane is filled with anti-C.diff. Toxin B is coated with antibody. During testing, the sample reacts with the anti-C.diff. Toxin B antibody-coated particle. The mixture is chromatographed by capillary action of the membrane and reacts with the anti-C.diff. Toxin B antibody and forms a coloured band. The presence of a coloured line in the line of the test band indicates a positive result, the absence of a coloured line indicates a negative result.
A coloured line in the control zone on all three test strips is a control of the procedure, indicating that the sample volume was adequate and the membrane was functioning properly.
