Examples of common causes of occult blood are: oesophagitis, gastritis, ulcers, gastrointestinal tract cancers (oesophagus, sto-mach, colon), haemorrhoids, ulcerative colitis, colonic polyps, diverticular disease, etc. For occult blood detection, both iFOBT and gFOBT use haemoglobin as the sole blood protein. The Diagnostik Nord HB-HP rapid occult blood test detects another blood protein complex, haemoglobin-haptoglobin, in addition to haemoglobin. As the haemoglobin-haptoglobin complex remains detectable in the digestive tract longer than haemoglobin, the detection of the double blood protein significantly increases the sensitivity of detection of occult blood from the upper gastrointestinal tract. If the test is performed correctly, the coloured C-line will always appear.
Principle:
The Hb+Hb-Hp Combined Rapid Test Form (Faeces) is an immunochromatographic test for the qualitative detection of haemoglobin and haemoglobin (Hb)-haptoglobin (Hp) complex from faeces.
The Hb-Hp Rapid Test Form
The membrane test line region is coated with anti-hemoglobin antibody During the test, the sample reacts with the anti-hemoglobin antibody coated particle. The mixture migrates up the membrane by capillary action and then reacts with the anti-hemoglobin antibody on the membrane, resulting in a coloured strip. The presence of a coloured stripe in the test line region indicates a positive result and the absence of a coloured stripe indicates a negative result. To verify the procedure, a coloured stripe appears in the control zone, confirming the correct functioning of the test sheet, the correct sample volume and the absorption of the sample.
